Tecan Austria Microplate Reader SPARK CYTO600

Cleaning Procedure with Ultrasonic Bath

Fill an ultrasonic bath with water and place a suitable beaker filled with distilled water into the ultrasonic bath.

Switch on the ultrasonic and immerse the lid of the NanoQuant Plate into the beaker, with bobbing movements for about 20 seconds. Take care not to immerse the hinge of the plate.

Repeat the procedure with the bottom part of the NanoQuant Plate.

Remove any surplus water from the NanoQuant Plate with dry and oil-free compressed air.

Cleaning Procedure with Kimwipe

Moisten a laboratory Kimwipe with 70% ethanol and clean the inner and outer surfaces of the NanoQuant Plate.

Moisten a piece of cotton or Kimwipe with distilled water and clean both sides of each quartz lens on the NanoQuant Plate.

Wipe off any excess liquid with a dry Kimwipe.

Rinse the fully lowered syringes with 70% EtOH for 30 minutes

Pump all the fluid from the syringe and tubing into a waste container

Rinse the syringe/injector system with 70% EtOH

Rinse the syringe/injector system with distilled or deionized water

Leave the fluid pathway filled for storage

Ensure all materials are available

Pipette 200μl of the reagent into the wells of a Greiner 96-well plate according to the plate layout

Calculate the Uniformity (CV%) using the formula: Uniformity (CV%) = (SDOG * 100) / meanOG

Is the calculated Uniformity (CV%) within acceptable limits?

Quality Control of the NanoQuant Plate

Ensure all materials are available

Pipette 2 μl of the reagent onto all positions of the NanoQuant Plate

Start the NanoQuant application and perform average blanking procedure over all wells (16 positions)

Detection Limit AlphaScreen Omnibeads 384-Well Plate

Switch on the instrument at least 15 minutes before starting a measurement

Prepare instrument for measurement

Dilute the Omnibeads stock solution 1:500 in PBS

Prepare 12 further dilutions in 1:2 steps

Pipette 100 μl of each dilution into 5 replicate wells of the microplate

Use 100 μl PBS for the blank wells

Calculate the average and standard deviation for each Omnibeads concentration

Wipe up the spill immediately with absorbent material

Dispose of contaminated material appropriately

Clean the instrument surfaces with a mild detergent

For biohazard spills, clean with B33 (Orochemie, Germany)

Switch off the instrument before removing any kind of spills