Tecan Austria Microplate Reader SPARK CYTO500

Ensure all materials are available

Pipette 200μl of the reagent into the wells of a Greiner 96-well plate according to the plate layout

Calculate the Uniformity (CV%) using the formula: Uniformity (CV%) = (SDOG * 100) / meanOG

Is the calculated Uniformity (CV%) within acceptable limits?

Quality Control of the NanoQuant Plate

Ensure all materials are available

Pipette 2 μl of the reagent onto all positions of the NanoQuant Plate

Start the NanoQuant application and perform average blanking procedure over all wells (16 positions)

Inspect the syringes(s) for leaks

Flush the whole system with distilled or deionized water after each use

Flush the whole system with distilled or deionized water when the syringe is not in use

Ensure syringes do not run dry for more than a few cycles

Ensure all materials are available

Adjust the cell suspension to a concentration of approximately 1x106 cells/ml

Perform manual counting of cell suspension using a Neubauer chamber

Pipette 10 μl of cell suspension into the counting chambers of a Tecan Cell Chip

Load the cell chip into the adapter at position 1

Start the Cell counting application

Cleaning Procedure with Ultrasonic Bath

Fill an ultrasonic bath with water and place a suitable beaker filled with distilled water into the ultrasonic bath.

Switch on the ultrasonic and immerse the lid of the NanoQuant Plate into the beaker, with bobbing movements for about 20 seconds. Take care not to immerse the hinge of the plate.

Repeat the procedure with the bottom part of the NanoQuant Plate.

Remove any surplus water from the NanoQuant Plate with dry and oil-free compressed air.

Cleaning Procedure with Kimwipe

Moisten a laboratory Kimwipe with 70% ethanol and clean the inner and outer surfaces of the NanoQuant Plate.

Moisten a piece of cotton or Kimwipe with distilled water and clean both sides of each quartz lens on the NanoQuant Plate.

Wipe off any excess liquid with a dry Kimwipe.

Detection Limit Top/Bottom 96-Well Plate

Ensure all materials are prepared

Prepare the instrument for measurement

Start the measurement immediately after pipetting

Pipette 200 μl for top measurement

Pipette 350 μl for bottom measurement

Calculate and record the detection limit (DL)

Uniformity Top/Bottom 96-Well Plate