VWR High Performance Pipettor 5000µL

Ensure the following parts are in place

Unscrew the connecting nut by turning it clockwise

Carefully separate the lower and upper parts

Remove the piston assembly, O-ring, and seal

Unscrew the connecting nut by turning it clockwise

Carefully separate the lower and upper parts

Remove the piston assembly and the seals

Clean, sterilize, or replace the tip-holder

Is lubrication of the piston and its seals necessary?

Disassemble the pipettor as described in chapter 12A - Maintenance.

Set aside the upper part in a clean, dry place.

Identify components that can be immersed in cleaning solution

Clean components using an ultrasonic bath (20 minutes at 50°C) or with a soft-cloth and brushes.

Degrease piston assembly and seals with isopropanol or ethanol before ultrasonic bath.

Use small round brushes with soft plastic bristles to clean the interior of the tip-holder.

Unscrew the connecting nut by turning it clockwise

Carefully separate the lower and upper parts

Remove the following components

Clean the piston with isopropanol or ethanol

Select the pipettor model for specific lubrication instructions

Fit pipettor with VWR Pipette Tips

Set pipettor to the maximum volume given in the specifications

Leak Test for 2µL to 200µL Single Channel Models

Aspirate the set volume from a beaker of distilled water

Maintain the pipettor in the vertical position and wait for 20 seconds

Did a water droplet appear at the end of the tip?

Re-immerse the tip below the surface of water. Did the water level inside the tip remain constant?