BD Biosciences Cell Sorter 662937A0

BD FACSymphony S6 cell sorter system daily shutdown procedure

Run the Clean Flow Cell command with the closed-loop nozzle installed

Fill the flow cell with cleaning solution

Put the lasers in standby and close the laser control software

Remove the nozzle and install the integrated closed-loop nozzle

Select Cytometer > Cleaning Modes > Clean Flow Cell

Warning: Handle the air filter and all instrument hardware as if capable of transmitting infection. Dispose of waste using proper precautions and in accordance with local regulations. Wear suitable protective clothing, eyewear, and gloves.

Slide open the sort collection chamber door.

Unscrew the screw at the top of the cover.

Pull off the cover and slide out the air filter.

Upload a photo of the removed air filter.

Bag the air filter to avoid exposure to biohazardous agents.

Install a new air filter in the door.

Make sure to install the filter with the grid side facing out so that it is visible through the closed door.

Reinstall the cover and tighten the screw.

Changing the disposable waste cap (see Emptying the Waste Container on page 137)

Empty the waste container daily and when the fluid indicator shows the waste is getting full. To prolong the life of the container, we recommend that you switch to the alternate container each time the waste is emptied.

To ensure that the appropriate solutions are dispensed, do not switch the tank positions. Make sure the label on each container matches the labeled port on the fluidics drawer.

All biological specimens and materials coming into contact with them can transmit potentially fatal disease. To prevent exposure to biohazardous agents, expose waste container contents to bleach (10% of total volume) before disposal. Dispose of waste in accordance with local regulations. Use proper precautions and wear suitable protective clothing, eyewear, and gloves.

Change the waste container cap every month, or when it has gotten wet, to prevent container pressurization. To order new replacement caps, contact your local BD Biosciences representative.

1 Disconnect the waste container’s sensor and fluid line connectors from the connector panel in the fluidics drawer.

The waste container can become pressurized when the cytometer is running. Always disconnect the container from the connectors panel before you empty it. Wait at least 1 minute for pressure to dissipate before you open the container.

2 Remove the disposable waste cap (large-sized cap) and attached trap from the container. Place the assembly on the bench label-side up.

Open the bleeder valve a small amount and leave it open until fluid seeps out through the valve.

If the fluid does not seep, close the valve, prime the fluid lines, and try again.

Wipe up any excess fluid that might have dripped into the fluidics drawer.

Place a small container under the bleeder valve to catch any fluid.

Slowly open the bleeder valve a small amount and leave it open until fluid seeps out through the valve.

Wipe up any excess fluid that might have dripped into the fluidics drawer.

Check the waste container and empty it if needed

Check the ethanol shutdown tank and refill it if needed

Select Cytometer > Fluidics Shutdown

Put the lasers in standby and close the laser control software

Remove the nozzle from the flow cell assembly