BD Biosciences Cell Sorter 662937A0

Check the waste container and empty it if needed

Check the ethanol shutdown tank and refill it if needed

Select Cytometer > Fluidics Shutdown

Put the lasers in standby and close the laser control software

Remove the nozzle from the flow cell assembly

BD FACSymphony S6 cell sorter system daily shutdown procedure

Run the Clean Flow Cell command with the closed-loop nozzle installed

Fill the flow cell with cleaning solution

Put the lasers in standby and close the laser control software

Remove the nozzle and install the integrated closed-loop nozzle

Select Cytometer > Cleaning Modes > Clean Flow Cell

Cleaning the Integrated Closed-Loop Nozzle

Lasers in standby and laser control software closed

Stream turned off and flow cell access door opened

Closed-loop nozzle removed from the cuvette flow cell

Nut on the side of the nozzle unscrewed and tubing removed

Nozzle sonicated for approximately 1 minute

Ferrule on the tubing and nut screwed back into the hole in the nozzle

Replacing the Tubing on the Closed-Loop Nozzle

Nut unscrewed from the closed-loop nozzle and from the union fitting and tubing removed

Changing the disposable waste cap (see Emptying the Waste Container on page 137)

Empty the waste container daily and when the fluid indicator shows the waste is getting full. To prolong the life of the container, we recommend that you switch to the alternate container each time the waste is emptied.

To ensure that the appropriate solutions are dispensed, do not switch the tank positions. Make sure the label on each container matches the labeled port on the fluidics drawer.

All biological specimens and materials coming into contact with them can transmit potentially fatal disease. To prevent exposure to biohazardous agents, expose waste container contents to bleach (10% of total volume) before disposal. Dispose of waste in accordance with local regulations. Use proper precautions and wear suitable protective clothing, eyewear, and gloves.

Change the waste container cap every month, or when it has gotten wet, to prevent container pressurization. To order new replacement caps, contact your local BD Biosciences representative.

1 Disconnect the waste container’s sensor and fluid line connectors from the connector panel in the fluidics drawer.

The waste container can become pressurized when the cytometer is running. Always disconnect the container from the connectors panel before you empty it. Wait at least 1 minute for pressure to dissipate before you open the container.

2 Remove the disposable waste cap (large-sized cap) and attached trap from the container. Place the assembly on the bench label-side up.

Warning: Use the Stop button in the laser control software to put the lasers in standby and then close the laser control software. This will turn off the laser diode, but will keep the diode temperature constant.

Slide the deflection plate removal tool behind one of the plates and pull straight out from the sort block.

Deflection plate removed successfully?

Sign off on the deflection plate removal