BioTek Instruments Microplate Dispenser Multiflo

Clean and rinse the supply bottles with deionized water before the first use, before each refill, and, periodically, as necessary, to prevent bacteria growth.

Empty the waste bottle often (at least daily), and firmly seat the waste bottle stopper.

Rinse the covers every time the wash or rinse bottles are filled.

Accumulated algae, fungi, or mold may require decontamination.

Do NOT autoclave the dual 8-tube and 32-tube dispense manifolds!

Note: The autoclavable 16-tube and non-autoclavable 8-tube manifolds have removable dispense tubes. We do not recommend routinely removing these tubes. In the case of a particularly difficult problem with any one channel, however, a tube may be removed and cleaned individually, or replaced.

Record number of plates processed with cassette

Remove protein residuals and fungi growth, (if necessary)

Daily maintenance involves flushing the dispensers with an appropriate reagent or deionized water throughout the day. Routine rinsing helps to prevent the tubes from clogging between runs.

Flushing the devices with deionized water is recommended at the end of the day for most applications.

The recommended rinsing frequency depends on the solutions currently in use:

32-tube-SB dispense manifolds are not clogged

Run S-DAY_RINSE_A&B without emptying the priming trough inserts

Lowered the manifolds into the priming trough?

Enter the volume of AutoPrime parameters

Enter the flow rate of AutoPrime parameters

If liquid has overflowed onto the plate carrier, transport rail, or platform, some buildup may occur and prevent the microplate from seating correctly on the carrier.

This can interfere with plate transport. Weekly cleaning is recommended.

2. Loosen the thumbscrew on the left side of the carrier to release the little bracket that holds the carrier in place and remove the carrier.

3. Clean the carrier, rails, and surface, using mild detergent and hot water, 70% isopropyl alcohol, or ethanol. Clean the priming trough, too.

4. If detergent was used, wipe the components with a cloth moistened with water. Use a clean, dry cloth to dry the components.

Clip the bottom bracket onto the transport rail, align the little bracket on the left rail to hold the carrier in place and tighten the thumbscrew.

Important! Solutions containing proteins, such as bovine serum albumin (BSA), will compromise the MultiFlo’s performance over time unless a strict maintenance regime is adhered to. Do not use isopropyl alcohol to flush out BSA.

When using protein solutions or similar fluids, BioTek recommends performing the following additional Maintenance procedures to thoroughly flush out protein particles and other contaminants from the fluid path.

Also note, some components can be autoclaved to sterilize them.

Four-liter volumes specified in the following are approximate amounts.

n S-DECONTAMINATE (S-DECON): this predefined protocol specifies Syringe A. Make a copy of the protocol and modify it for Syringe B.;