Eppendorf CO2 Incubator Shaker S41i

 Auto-Zero the CO2 system at least once monthly to ensure that the CO2 level is correct.

 We recommend monthly replacement of the water in the humidity trays. Clean the trays at the same time.

 If required, you can take a sample of the gas inside the chamber using the CO2 sample port, and check it using a CO2 gas analyzer (see CO2 Sampling with Analyzer on p. 62).;

1. Check that the temperature and CO2 levels are reading within specification.

2. Check that gas supply lines have inline regulators, that they are properly installed and functioning.

3. Check the reserve pressure in the CO2 cylinder (normally 725 psi or 50 bar when full). The design of the incubator/shaker ensures very low consumption of CO2: during normal working conditions, a typical large cylinder should last approximately 12 months (frequent door openings will deplete the supply more rapidly).

If there is a significant drop at the cylinder pressure of 725 psi or 50 bar, it means that the cylinder is almost empty and should be replaced. Ensuring that there are no leaks at any of the connections will ensure a greater lifetime to the CO2 supply and will help avoid accidentally running out of CO2.

4. Check the ALARM and EVENT LOG screens for any alarms or events that may have occurred overnight.;

 If you are using them for humidification, check the humidity trays and refill them as needed. Be careful not to exceed the maximum volume of 250 mL per pan. The use of warm water (~ 37.0 °C) will ensure a rapid return to optimum chamber conditions.;