Eppendorf CO2 Incubator 48S

1. Check that the temperature and CO2 levels are reading within specification.

2. Check the reserve pressure in the CO2 cylinder (normally 725 psi or 50 bar when full). The design of the incubator ensures very low consumption of CO2: during normal working conditions, a typical large cylinder should last approximately 12 months (frequent door openings will deplete the supply more rapidly, however).

If there is a significant drop at the cylinder pressure of 725 psi or 50 bar, it means that the cylinder is almost empty and should be replaced. Making certain that there are no leaks at any of the connections will ensure a greater lifetime to the CO2 supply and will help avoid accidentally running out of CO2.

3. Any spills in the chamber should be cleaned immediately to protect the stainless steel surfaces.;

Remember that we recommend routine replacement of the water in the humidity tray, and that you clean the tray at the same time.

1. If you are not changing the humidity tray water and cleaning the humidity tray regularly, you should at least fill the humidity tray once a month. Do not, however, exceed the maximum volume of 0.5 liters. Use warm water (~37.0°C) to ensure a rapid return to optimum chamber conditions. After adding water, check that the humidity tray is centered between the shelf rack supports.

2. If required, you can take a sample of the gas inside the chamber using the CO2 sample port, and check it using a CO2 gas analyzer. The CO2 sample port is located at the top of the rear panel.

If you conduct a sampling, please do the following:

• Turn off the CO2 gas by re-programming the setpoint for CO2 to 0.0% to prevent CO2 from being injected into the chamber and giving a false reading.

• Use a flow rate that is ≤ 0.5 liters/minute to take a sample.

• Keep the door closed to minimize heat loss.

• Remember to reset the CO2 setpoint to the desired level after sampling.; We recommend that you perform a CO2 AutoZero prior to sampling (see Section 3.5).

3. We also recommend that you AutoZero the CO2 system at least once every 28 days to ensure that CO2 level is correct.;